The goal of this clinical trial is to develop an in vitro model based on human duodenal organoids for the study of interactions between the human intestinal epithelium and enteric pathogens. Patients subjected to surgical removal of a portion of the duodenum for clinical indications, with availability of histologically normal duodenal tissue, will be asked to donate residual biological material for research purposes. The model generation procedure includes isolating intestinal stem cells from duodenal tissue, generating 3D intestinal organoids, expanding and differentiating the organoids, and inducing epithelial polarisation and maturation through air-liquid interface culture. Fully differentiated organoids will be used in in vitro co-culture experiments to assess pathogen viability, as well as the development of host epithelial responses to pathogens or their derivatives. Transcriptomic and proteomic data will also be obtained.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
6
Donation of residual biological samples for the development of duodenal organoids that will be tested for the study of host-intestinal pathogen interactions
IRCCS Ospedale Sacro Cuore Don Calabria
Negrar, Verona, Italy
RECRUITINGSuccessful generation of stable and expandable human duodenal organoids from duodenal tissue
Time frame: Through study completion, an average of 18 months
Determination of modifications in pathogen morphology, development, motility and viability induced by the maintenance in the model
Time frame: Through study completion, an average of 18 months
Determination of functional changes and gene expression modulation induced in human the human epithelial compartment following co-culture with the enteric pathogens
Time frame: Through study completion, an average of 18 months
Determination of functional changes and gene expression modulation induced in the human epithelial compartment following exposure to pathogen derivatives
Time frame: Through study completion, an average of 18 months
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