Metabolic diseases, including childhood obesity, represent a major public health challenge. Studies in mice have identified transcriptomic markers in adipose tissue capable of predicting a predisposition to obesity prior to any weight gain. The MARIOBE project aims to translate this approach to young children (≤ 4 years old) in order to identify individual vulnerability to obesity at an early stage.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
SCREENING
Masking
NONE
Enrollment
400
during a scheduled surgery before the age of 4 years, collection of a blood sample (2.5 mL in a PAXgene tube) and two adipose tissue fragments, each measuring 5 mm x 5 mm x 5 mm. tracking of weight and height curves from birth to age 6
CHU de Clermont-Ferrand
Clermont-Ferrand, France
transcriptomic markers in children under 4 years of age that predict a predisposition to being overweight at age 6 (BMI > IOTF25).
Ranking of the discriminative capacity of adipocyte and blood transcripts before 4 years of age to predict the development of overweight (BMI \> IOTF25) at age 6, using Partial Least Squares Discriminant Analysis (PLS-DA).
Time frame: perioperative
analyze weight trajectories
Selection and validation of transcriptomic markers for the development of a predictive score for the risk of overweight and obesity in children
Time frame: at age 6 years
molecular and epigenetic mechanisms associated with the identified transcriptomic signatures.
methylation profiles
Time frame: at the time of blood ans adipose tissu sampling
comparison of transcriptomic expression levels between blood and adipose tissue
percentage of common markers sampled from blood and adipose tissue
Time frame: perioperative
develop a quantitative test based on digital PCR to define individual expression thresholds predictive of risk
Performance of the digital PCR test evaluated by the ability to discriminate weight trajectories (AUC of ROC curves).
Time frame: at age 6 years
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