The purpose of this study is to explore pro-metastatic changes in breast cancer (BC) associated with the stiffness of biopsy site markers. This study aims to determine whether the stiffness of a biopsy site marker promotes inflammatory changes in breast cancer and to evaluate whether there is a relationship between biopsy site marker stiffness and inflammation.
This prospective study aims to evaluate whether anti-inflammatory and pro-metastatic changes at the post-biopsy site are associated with the type of biopsy-site marker used, with particular focus on marker stiffness. Tissue samples will be collected from breast cancer patients who received a biopsy-site marker at the time of diagnosis.
Study Type
OBSERVATIONAL
Enrollment
140
Tissue samples will be collected from Breast Cancer patients, stages I-III, who underwent lumpectomy (mastectomy) after biopsy site marker placement during image-guided biopsy.
OU Health Stephenson Cancer Center
Oklahoma City, Oklahoma, United States
RECRUITINGProportion of Patients With Evaluable Tissue Samples.
To process 100 surgically resected breast tumors that had needle biopsy and biopsy site marker placement outside OKC downtown campus.
Time frame: 1 year
Differences in Stiffness of Tissue Samples Collected During Surgical Biopsy.
We will conduct a spatiotemporal analysis of surgically resected tissues to evaluate whether the stiffness of the chemical materials is associated with an anti-inflammatory microenvironment and a higher prevalence of epithelial-mesenchymal transition (EMT) in cancer cells. To better characterize these differences, we will assess phenotypic changes using multiplex immunofluorescence on resected surgical specimens with the biopsy site marker in place, enabling precise spatial localization and comparison of tissue responses over time.
Time frame: 1 Year
Differences in Gene Expressions From Surgically Resected Tissue in Comparison To Those Associated With EMT.
A digital spatial transcriptomic analysis will be performed to evaluate unbiased, global gene expression changes beyond those related to EMT. This analysis will focus specifically on the materials used in the biopsy-site markers and their relative stiffness. In parallel, we will assess whether the stiffness of the implanted markers is associated with the development of an anti-inflammatory tumor microenvironment.
Time frame: 1 Year
Number of Different Variations In Gene Expression From Surgically Resected Tissue Samples.
Using spatial transcriptomic technology, we will assess variations in gene expression within surgically resected tissue and compare these profiles to baseline gene expression levels observed in adjacent normal tissue.
Time frame: 1 Year
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