The goal of this clinical trial is to evaluate the effects of low-level light therapy on cellular energy and oxidative stress in healthy adults and patients with Type 1 diabetes. The main questions it aims to answer are: Does light therapy change adenosine triphosphate (ATP) levels and cytochrome c oxidase activity in blood cells? How does the intervention affect oxidative stress markers, specifically glutathione and malondialdehyde concentrations? Researchers will compare blood samples collected before the light therapy to those collected afterward to see if the intervention alters mitochondrial function. Researchers will also compare the responses of healthy volunteers to those of diabetic patients. Participants will: Provide a baseline blood sample. Receive red and near-infrared light exposure (660 nm and 850 nm) on their arms for 10 to 20 minutes using a therapy pad. Provide a final blood sample immediately after the light exposure.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
30
Participants receive a single photobiomodulation session using a flexible light therapy pad. The device delivers red light at 660 nm and near-infrared light at 850 nm with an operating voltage of 5V and a working current of 1.7A. The pad is placed in direct contact with the upper arm of each participant for a timed duration of 10 to 20 minutes. Researchers evaluate cellular responses before and immediately after this single application.
Change in Adenosine Triphosphate (ATP) Concentration
Researchers measure ATP levels in blood cells using a specific assay kit to evaluate cellular energy production.
Time frame: Baseline and immediately after the 10-to-20-minute light therapy session
Change in Cytochrome c Oxidase Activity
Researchers assess the specific activity of cytochrome c oxidase in blood samples via a biochemical assay to determine mitochondrial function.
Time frame: Baseline and immediately after the 10-to-20-minute light therapy session
Change in Malondialdehyde (MDA) Levels
Researchers quantify MDA concentration in the collected blood samples to assess oxidative stress.
Time frame: Baseline and immediately after the 10-to-20-minute light therapy session
Change in Lactate and Pyruvate Concentrations
Researchers determine the concentrations of lactate and pyruvate in blood cells to analyze metabolic shifts.
Time frame: Baseline and immediately after the 10 to 20-minute light therapy session
Change in Glutathione (GSH) Levels
Researchers measure glutathione concentration in the blood samples to evaluate antioxidant capacity.
Time frame: Baseline and immediately after the 10-to-20-minute light therapy session
Change in Blood Glucose Levels
Researchers measure blood glucose concentrations in the collected blood samples to evaluate glycemic changes following the light therapy session.
Time frame: Baseline and immediately after the 10-to-20-minute light therapy session
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.