The purpose of this research is to find out how a nasal spray (fluticasone furoate), sometimes given to children with obstructive sleep apnea syndrome (OSAS), works on certain cells within a child's adenoids. We hypothesize that intranasal steroids lead to an upregulation of T regulatory cells in the adenoid tissues of children with OSAS. This will result in a local reduction in inflammation and edema explaining the improvement in OSAS.
The objective was to determine the effect of intranasal corticosteroid therapy on T-regulatory cells and other inflammatory cytokines in adenoid tissues in children with obstructive sleep apnea syndrome.Children were randomized to either no treatment or treatment with fluticasone furoate nasal spray, 55 μg/nostril daily, for 2 weeks before adenotonsillectomy. Adenoid tissue was obtained at the time of the procedure.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
24
treatment with fluticasone furoate (55 mcg/nostril once daily) for 2 weeks prior to adenotonsillectomy
University of Chicago
Chicago, Illinois, United States
Number of CD25 Pos/FoxP3 Positive Cells
The number of tissue T-regulatory cells, as determined by staining with FOXP3, CD4, and CD25
Time frame: following adenoidectomy (2 weeks)
Number of CD4 Pos/FOXP3 Positive Cells
The number of tissue T-regulatory cells, as determined by staining with FOXP3, CD4, and CD25
Time frame: following adenoidectomy (2 weeks)
IL-10 Staining Intensity
IL-10 staining intensity on immunohistochemical staining of adenoid tissues. Units are Integrated optical density (IOD)/100 micrometer squared.
Time frame: following adenoidectomy (2 weeks)
Amount of IL-10 Secreted by Adenoid Cells After PHA Stimulation
Amount of IL-10 secreted by adenoid cells after PHA stimulation
Time frame: following adenoidectomy (2 weeks)
Amount of TGF Secreted by Adenoid Cells After PHA Stimulation
Amount of TGF secreted by adenoid cells after PHA stimulation
Time frame: following adenoidectomy (2 weeks)
Adjusted Volume of the Removed Adenoids
To adjust for different weights of the children, the volume of the adenoids, estimated by water displacement in the operating room in mL, was divided by the respective weights (kg) of the patients and multiplied by 100.
Time frame: following adenoidectomy (2 weeks)
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