Pharmacokinetic and Dose Response Study of Asfotase Alfa in Adult Patients With Pediatric-Onset Hypophosphatasia (HPP)

Inclusion Criteria: 1. Participants or their legal representative(s) provided written informed consent prior to undergoing any study-related procedures. 2. Participants were ≥18 years of age at Screening. 3. Participant had pediatric-onset hypophosphatasia (HPP), defined as onset of first sign(s)/symptom (s) of HPP prior to 18 years of age. 4. Participants had a documented diagnosis of HPP as indicated by a documented history of HPP-related skeletal abnormalities and 1 or more of the following: * Documented tissue-nonspecific alkaline phosphatase (TNSALP) gene mutation(s) from a certified laboratory. * Serum alkaline phosphatase (ALP) level below the age-adjusted normal range AND plasma pyridoxal-5'-phosphate (PLP) above the upper limit of normal at Screening. 5. Participants had a plasma inorganic pyrophosphate (PPi) level of ≥3.9 micromolar (µM) at Screening. 6. Female participants of childbearing potential had a negative pregnancy test at the time of enrollment. 7. Sexually active male and female participants of childbearing potential agreed to use a highly effective method of birth control during the study. 8. Female participants not of child-bearing potential due to sterilization (at least 6 weeks after surgical bilateral oophorectomy with or without hysterectomy or at least 6 weeks after tubal ligation) confirmed by medical history, or menopause. 9. Participants were willing to comply with study procedures and the visit schedule. Exclusion Criteria: 1. Investigational site personnel directly affiliated with this study and/or their immediate families. Immediate family was defined as a spouse, parent, child, or sibling, whether biological or legally adopted. 2. Employees of Alexion Pharmaceuticals. 3. Currently enrolled in a clinical study involving another study drug or non-approved use of a drug or device. 4. Participated, within the last 30 days, in a clinical study involving a study drug (other than the study drug used in this study). 5. Completed or withdrawn from this study or any other study investigating asfotase alfa in the previous 3 years. 6. Women who were pregnant, planning to become pregnant, or breastfeeding. 7. Serum 25-hydroxy Vitamin D levels below 20 nanogram (ng) per milliliter (mL) at Screening. 8. Screening serum creatinine or parathyroid hormone (PTH) levels ≥1.5 times the upper limit of normal. 9. Any medical condition, serious concurrent illness and/or injury, recent orthopedic surgery, or other extenuating circumstance that, in the opinion of the Investigator, may have significantly interfered with study compliance or study endpoints. 10. Prior treatment with bisphosphonates within 2 years of study entry for any length of time or for more than 2 consecutive years at any prior timepoint. 11. Treatment with PTH, strontium, or sclerostin inhibitors within 6 months prior to the first dose of study drug. 12. Unwilling or unable to comply with the use of a data collection device on which study participants directly recorded data.