The Effects of Age on Wound Healing on Free Gingival Graft Surgery in the Treatment of Attached Keratinized Gingival Deficiency by Determining Clinical and Biochemical Outcomes

N/AActive Not RecruitingNCT06749119

Marmara University30 enrolled

Overview

This study aimed to assess the effects of age on wound healing on free gingival graft (FGG) surgery in the treatment of attached keratinized gingival deficiency by determining clinical and biochemical outcomes.

Aim of this study was to evaluate the effect of age on clinical and biochemical healing outcomes on FGG surgery in the treatment of attached keratinized gingival deficiency (≤2 mm) at mandibular anterior region. A total of 30 systemically healthy patients, divided into two groups and planned for FGG operations, were included in this study. These groups are; Group l : 15 patients in the age range of 20-35, group 2: 15 patients in the age range of 50-65. Gingival crevicular fluid and wound fluid samples were taken from these patients to analyze VEGF, EGF and TGF-B1 levels by ELISA. Wound fluid samples were taken baseline, 4,7, and 14 days after surgery. Clinical parameters included measurement of probing depth (PD), clinical attachment level (CAL), bleeding of probing (BOP), plaque index (PI), gingival index (GI) at 6 sites per tooth except the third molars were measured at baseline and l, 3 and 6 months after surgery. Keratinized gingiva width, keratinized gingiva thickness of the operation sites were measured baseline and 1,3 and 6 months after surgery. Vertical dimension of the graft, horizontal dimension of the graft, graft thickness were measured at baseline and 1,3,6 months after surgery. Graft area measurements were calculated on standard photographs by a Java-based analysis program (ImageJ, National Institutes of Health, Bethesda, MD) to determine the shrinkage rate of the grafts. The graft shrinkage ratio was calculated as the difference between baseline to 1-month, baseline to 3-months, baseline to 6-month graft site value. Data were analyzed using appropriate statistical tests.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

TREATMENT

Masking

SINGLE

Enrollment

Conditions

Wound Healing Tissue Grafts Age Vegf TGF-β

Interventions

Free gingival graft operationPROCEDURE

Free gingival graft was harvested from palatal region and stabilized on the maxillary anterior recipient bed with sutures .

Eligibility

Sex: ALLMin age: 20 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * having ≤2mm of keratinized gingiva in at least 1 mandibular anterior tooth * periodontally healthy with full-mouth bleeding and plaque score ≤ 10% * no use of any drugs that will affect periodontal tissue health and healing process * absence of any systemic condition or illness Exclusion Criteria: * smokers * previous periodontal surgery * a pathology in the apical region of the tooth * pregnancy, lactation

Locations (1)

Marmara University Faculty of Dentistry

Istanbul, Maltepe, Turkey (Türkiye)

Outcomes

Primary Outcomes

Shrinkage percentage (%)

Shrinkage percentage of the graft area due to the changes in graft dimensions

Time frame: from baseline to 1-,3-,6- months

Secondary Outcomes

VEGF levels (pg/ml)

change in wound fluid VEGF levels from baseline to 1-,3-,6- months after surgery

Time frame: from baseline to 1-,3-,6- months

EGF levels (pg/ml)

change in wound fluid EGF levels from baseline to 1-,3-,6- months after surgery

Time frame: baseline to 1-,3-,6- months

TGFβ-1 levels (pg/ml)

change in wound fluid TGFβ-1 levels from baseline to 1-,3-,6- months after surgery

Time frame: from baseline to 1-,3-,6- months

Data from ClinicalTrials.gov

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